What is Ercc Spike?
ERCC ExFold RNA Spike-In Mixes are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms.
What is spike-in normalization?
Spike-in normalization will preserve changes in total RNA content between cells, whereas non-DE methods will treat such changes as bias (as a majority of genes are affected) and remove them.
What is Ercc normalization?
We propose a normalization strategy, called remove unwanted variation (RUV), that adjusts for nuisance technical effects by performing factor analysis on suitable sets of control genes (e.g., ERCC spike-ins) or samples (e.g., replicate libraries).
What is Ercc?
ERCC RNA Spike-In Control Mixes. Purpose. The Ambion® ERCC RNA Spike-In Control Mixes provide a set of external RNA controls that enable performance assessment of a variety of technology platforms used for gene expression experiments.
What is the use of ERCC?
Ambion® ERCC RNA Spike-In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms.
What is a spike-in?
A spike-in is designed to bind to a DNA molecule with a matching sequence, known as a control probe. This process of specific binding is called hybridization. A known quantity of RNA spike-in is mixed with the experiment sample during preparation.
How do you normalize with Spike?
Simply add the Spike-in Chromatin and Spike-in Antibody to your standard ChIP reaction containing sample chromatin and the antibody of interest. Use the recommended ratio based on the antibody target. Perform ChIP as normal. Follow the guidelines to normalize the data for qPCR or ChIP-Seq analysis.
How does DESeq2 normalize?
DESeq2 performs an internal normalization where geometric mean is calculated for each gene across all samples. The counts for a gene in each sample is then divided by this mean. The median of these ratios in a sample is the size factor for that sample.
Who is the founder of ERCC?
The Evangelical Reformed Church of Christ for the purpose of training evangelists and pastors to reach the unreached. The school was started by Rev. W. Desmond Douglas who was actually the sixth field superintendent of the Sudan United Mission (SUM) a name the church retained from 1916- 1956.
What is an example of a spike?
An example of to spike is to secure a tent to the ground with a stake. An example of to spike is to add vodka to the punch. The definition of a spike is a piece of something that is thick and long with a pointed and sharp end, usually made of metal.
What is a spike solution?
A spike solution, or spike, is a technical investigation. It’s a small experiment to research the answer to a problem. For example, a programmer might not know whether Java throws an exception on arithmetic overflow. A quick ten-minute spike will answer the question.
What is Rlog transformation?
Both variance stabilizing transformation (VST) and regularized log transformation (rlog) aim to remove the dependence of the variance on the mean.